1 . This is my sister.(变为否定句)     
This ________ ________ sister.

2 . Jane is helping spread the message.(改为否定句)
Jane ________ ________ spread the message.

3 . That is a Japanese flag.(变否定句)
________ ________ a Japanese flag.

4 . —________ your sister spell English?
—Sorry, she ________.

A．Can；can’t

B．Does；does

C．Is；isn’t

5 . at the front of...     1    
there isn’t/aren’t...     2    
in your class    3    
on the wall    4    

6 . The boy is playing basketball.（改为否定句）
The boy ______ _______ basketball.

7 . There is some dirty water in the swimming pool.（改为否定句）
There ________ ________ dirty water in the swimming pool.

8 . Li Ming is going to take part in the competition this weekend. (改为否定句)
Li Ming ______   ______ to take part in the competition this weekend.

9 . Is Amy tall and slim? （作否定回答）
No, ________ ________.

10 . There ________ (not be) any milk in the bottle.